Department of Forest Genetics and Plant PhysiologyThe Department of forest genetics and plant physiology is part of Umeå Plant Science Centre (UPSC, https://www.upsc.se/) which is a centre of excellence for experimental plant research and forest biotechnology in Northern Sweden. Our mission is to perform excellent and innovative basic research and generate knowledge that benefits forestry, agriculture, environment and society. We work across a wide range of disciplines in plant science reaching from cell biology to ecophysiology and from basic research to industrial applications. Our common goal is to understand the plants’ ability to grow, adapt and acclimate to a changing world and how we can breed better plants.About the positionThe research activities will focus on studying the role of the plant hormone cytokinin in controlling adventitious root development in Arabidopsis thaliana. Interconnected transcriptional and hormonal networks control adventitious root initiation, but the underlying genetic and molecular bases orchestrating these networks have long remained unclear. Several Arabidopsis Response Regulators (ARRs) transcription factors involved in the cytokinin signaling pathway have been identified and shown to control adventitious root initiation. The objective is to study the role of these transcription factors in the repression of AR initiation.As a researcher, you will perform ChIP-qPCR experiments to identify and confirm downstream targets of the ARR transcription factors. You will clone the coding sequences in an expression vector, purify the ARR proteins, and perform EMSA experiments to confirm the interaction with the target promoters. You will characterize mutants affected in the expression of the downstream targets to identify the elements of th…Forskare, cell- och molekylärbiologiCell- och molekylärbiologer m.fl.
Jobbfinder